Characterization of Monoclonal Antibodies for Prostate-specific Antigen and Development of Highly Sensitive Free Prostate-specific Antigen Assays
نویسندگان
چکیده
منابع مشابه
Characterization of monoclonal antibodies for prostate-specific antigen and development of highly sensitive free prostate-specific antigen assays.
BACKGROUND The recent elucidation of the importance of serological free prostate-specific antigen (PSA) in the diagnosis of prostate cancer has created a demand for immunoassays specific for free PSA. METHODS We developed and characterized 11 monoclonal antibodies with high affinities for PSA (Ka values from 1.1 x 10(8) to 1.8 x 10(10)L/mol), only 3 of which cross-react with human glandular k...
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Background: It is relevant to highlight that there is not a precise and perfect report on either 95 percentile value (upper limit of normal range) or on appropriate reference intervals for serum PSA in Iranian population. Objective: To determine age-specific reference ranges for serum prostate-specific antigen (PSA) concentration and PSA density (PSAD) and prostate volumes in a population of he...
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Background: Immunoradiometric assay is one of the most common and precise methods for determination of prostate specific antigen (PSA) in clinical laboratories. Usual use of human serum in routine assays has many disadvantages such as easy contamination and precipitation, instability and unavailability. Thus in order to avoid these problems the artificial matrix was used which acts sim...
متن کاملproduction and characterization of monoclonal antibodies against human prostate specific antigen
background: prostate specific antigen (psa) is an important laboratory marker for diagnosis of prostatic cancer. thus, development of diagnostic tools specific for psa plays an important role in screening, monitoring and early diagnosis of prostate cancer. in this paper, the production and characterization of a panel of murine monoclonal antibodies (mabs) against psa have been presented. metho...
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Introduction: Prostatic adenocarcinoma is one the most common malignances and the second cause of death in males due to cancer. Prostate Specific Antigen (PZSA) serum level is the first marker to screen such patients. We aimed in this study, to assess the intensity of prostatic adenocarcinoma tissue staining with PSA staining method. Methods: In this corss-sectional study, prostatic adenocarcin...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1999
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/45.3.347